[Use of thrombin generation assay for the evaluation of coagulation and anticoagulant activity of hemostasis system in patients with abdominal sepsis].

Generally recognized factor, which complicates the course of sepsis, is the development of hypercoagulation syndrome. The increase of thrombin coagulation indicates on the elevation of risk of thrombus formation in microcirculation vessels, which could cause the formation of multiple organ failure. The thrombin generation assay is a new method of the evaluation of homeostasis system status.

[The conditions of arrangement of the thrombin generation test to detect the hypercoagulation status].

The study covered the impact of modes of preparation of plasma samples to arrange the thrombin generation test for the purpose to establish adequately the hypercoagulation status. The optimal regimen is determined to prepare the samples to be used in the study. The group of females was involved into the study to take the composite oral contraceptives to demonstrate the possibility to apply the thrombin generation test to reveal the hypercoagulation.

[Experimental quality assessment of APTT-control reagent kits in the study of plasma with varying Factor VIII activity].

An investigation into activated partial thromboplastin time (APTT) in the plasma samples with varying Factor VIII activities has determined the following parameters of the quality of APTT-control reagent kits: coefficient of variation, linearity, sensitivity, and detection. The kit is shown to meet GOST P 51352-99 requirements. The determination of the validity coefficient of approximation has proven a directly proportional functional relationship of the APTT values to the Factor VIII activity in the bilogarithmic coordinate system.

[Experimental quality assessment of APTT-control reagent kits in the study of heparin-containing plasma in therapeutic ranges].

Investigation of activated partial thromboplastin time (APTT) in the plasma samples containing different levels of heparin determines the following parameters of the quality of APTT-control reagent kits: coefficient of variation, linearity, sensitivity, and detection. The kit is shown to meet GOST P 51352-99 requirements. The determination of the validity coefficient of approximation has proven a directly proportional functional relationship of the APTT values to the level of heparin in therapeutic ranges in the logarithmic coordinate system.

[The state of oxidant-antioxidant system in basal and latent hyperhomocysteinemia in patients with atherosclerosis of the lower extremity arteries].

The diagnostics of basal and post-load (latent) hyperhomocysteinemia (HHC) was made in 110 patients with atherosclerosis of lower extremity arteries. Basal HHC was found in 61% of the patients. Latent HHC was found when using a methionine load test in 20 (47%) out of 43 patients without basal HHC.