Sleep-wakefulness cycle and behavior in pannexin1 knockout mice.

Pannexins are membrane channel proteins that play a role in a number of critical biological processes (Panchin et al., 2000; Shestopalov, Panchin, 2008). Among other cellular functions, pannexin hemichannels serve as purine nucleoside conduits providing ATP efflux into the extracellular space (Dahl, 2015), where it is rapidly degraded to adenosine.

Effects of striatal transplantation of cells transfected with GDNF gene without pre- and pro-regions in mouse model of Parkinson's disease.

Background: Previously, we have shown that transgenic cells bearing the GDNF gene with deleted pre- and pro-regions (mGDNF) can release transgenic GDNF. The medium conditioned by transgenic cells with mGDNF induced axonal growth in rat embryonic spinal ganglion in vitro. Here we demonstrate a neurotrophic effect of mGDNF on PC12 cells in vitro as well as its neuroprotective effect on dopaminergic neurons in the substantia nigra pars compacta in vivo as indicated by improved motor coordination and sleep-wakefulness cycle in the MPTP mouse model of Parkinson's disease.

[Dependence of Accuracy of Automatic Sleep Scoring on Spectral Characteristics of Mice EEG].

Computer programs for automatic sleep scoring of human and animals EEG records are widely used in many branches of physiological research. They are particularly useful during the sleep research because the traditional methods requiring human expert scoring of long records are very laborious and time-consuming. The aim of this work was to investigate the dependency of accuracy of automatic sleep scoring on different EEG parameters for EEG-recording quality assessment.

[Early Stages of Parkinson's Disease: Comparative Characteristics of Sleep-Wakefulness Cycle in Patients and Model Animals].

The results of study of sleep-wakefulness cycle in experimental models of pre-clinical and early clinical stages of Parkinson's disease present and compared to some clinical examples. The conclusion is, the increase in activity level and decrease in total amount of slow wave and paradoxical sleep in model animals are taking place at the same circadian period of the secretion of pineal melatonin as sleep disorders in patients.

The effect of hydrogen sulfide on electrical activity of rat atrial myocardium.

Changes in the configuration of action potentials and in the frequency of pacemaker discharges in a preparation of isolated rat right atrium under the effect of sodium hydrosulfide degrading in water solution with hydrogen sulfide release were studied by intracellular recording of action potentials in the myocardium. Sodium hydrosulfide in concentrations of 100-500 microM markedly reduced the duration of action potentials at the level of 50 and 90% repolarization and decelerated the sinus rhythm. Moreover, sodium hydrosulfide shortened action potentials in the preparations working in the forced rhythm.