Evaluation of the efficacy of granulocyte colony-stimulating factor for the treatment of experimental myocardial destruction in mice.

We studied the effects of recombinant granulocytic CSF on heart remodeling in BALB/c mice after cryodestruction. Administration of granulocytic CSF was started 1 day after cryodestruction (subcutaneously, 10 μg/kg/day, for 4 days). As early as after the first injection, leukocytosis in the peripheral blood started to develop, leukocyte count peaked on days 4-6 and returned to normal on day 14.

Comparative assessment of heart remodeling in rats after experimental coronary stenosis and cryodestruction.

Comparative analysis of myocardial changes due to heart remodeling after experimental coronary stenosis and cryodestruction in rats was performed. Similar picture of heart remodeling was observed in all animals on day 45 irrespective of the type of destruction: hypertrophy of intact myocardium of the left ventricle, formation of extensive connective tissue cicatrix, and similar structural changes in the myocardium adjacent to the damage area. The type of the damaging influence possibly plays a role at the stage of lesion formation.

Effect of transplantation of bone marrow cells on morphology of rat myocardium after cryodestruction.

We studied the effects of bone marrow cell transplantation on myocardium of the prenecrotic zone in Wistar rats. Intramyocardial cell transplantation reduced the severity of hypertrophy of myocardium and the degree of its cicatricial degeneration on day 40 after cryodestruction. The morphology of the myocardium in the prenecrotic zone depended on the type of transplanted cells.

[Possible role of sarcoplasmatic reticulum in anti-arrhythmic effects of the class III agent amiodarone].

The study was designed to evaluate changes in functional activity of cardiomyocyte sarcoplasmic reticulum under effect of amiodarone (1 mM). It was shown that post-rest potentiation significantly increased after perfusion of cardiomyocytes with amiodarone or calcium (3 mM). The stimulatory action of amiodarone was more pronounced than that of calcium.

Effect of stress-proteins on survival of bone marrow mesenchymal stem cells after intramyocardial transplantation against the background of postinfarction heart remodeling.

We studied the presence of colony-forming cells in cell culture from rat heart 40 days after experimental myocardial infraction. The mean cellularity in this pathology was 12+/-8 cell/cm2, which is 20-fold lower than in intact myocardium. Transplantation of mesenchymal stem cells into the remodeling myocardium restored the pool of colony-forming cells.