Staphylococcus simulans Recombinant Lysostaphin: Production, Purification, and Determination of Antistaphylococcal Activity.

Staphylococcus simulans lysostaphin is an endopeptidase lysing staphylococcus cell walls by cleaving pentaglycine cross-bridges in their peptidoglycan. A synthetic gene encoding S. simulans lysostaphin was cloned in Escherichia coli cells, and producer strains were designed.

[Persistence of Pseudomonas aeruginosa strains in patients of Federal Scientific Center of Transplantology and Artificial Organs].

Aim: Study genetic diversity of P. aeruginosa strains persisting in patients of Federal Scientific Center of Transplantology and Artificial Organs, and main factors facilitating persistence of strains in the hospital.

Materials And Methods: 136 P.

[Burkholderia cepacia persistence in patients with mucoviscidosis].

Aim: Study features of persistence of Burkholderia cepacia in mucoviscidosis patients.

Materials And Methods: In the period from 2008 to 2009, 56 B. cepacia strains isolated from children with mucoviscidosis were obtained.

[Genetic diversity of Staphylococcus aureus strains that colonize nose mucous membrane of children living in a large industrial city].

Aim: Study of genetic diversity of Staphylococcus aureus strains that colonize nose mucous membrane of children living in an ecologically unfavorable district of Krasnoyarsk.

Materials And Methods: 20 S.aureus strains isolated from mucous membrane of anterior part of nose of children were genotyped by RAPD-PCR (random ampliphied polymorphic DNA) with an oligonucleotide primer (10 Sh1 nucleotide).

[Microbial population of lower respiratory tract in children from different age groups with cystic fibrosis].

Aim: To study microflora of lower respiratory tract of children from different age groups with cystic fibrosis during follow-up for determination of its variability and possible sources of infectious complications.

Materials And Methods: One hundred forty-one medical histories of patients from different age groups with cystic fibrosis living in various regions of Russian Federation were analyzed. Eighty-four children with cystic fibrosis living in Moscow and Moscow region treated as outpatients and inpatients were prospectively followed.