Development of early diagnosis of Parkinson's disease on animal models based on the intranasal administration of α-methyl-p-tyrosine methyl ester in a gel system.

The fight against neurodegenerative diseases, including Parkinson's disease (PD), is a global challenge of this century. The effectiveness of current PD therapy is limited, since it is diagnosed many years after the onset, following the death of most nigrostriatal dopaminergic neurons regulating motor function. PD treatment could be greatly improved if it was started at an early (preclinical) stage.

Alternative approach to the treatment of diabetes mellitus.

The influence of insulin preparations (Actrapid and Ransulin) on the glucose and insulin blood level has been studied in patients with diabetes mellitus. It has been shown that comparable changes in the measured parameters are achieved in most patients with oral doses of Ransulin that are two to three times higher than the doses of Actrapid.

[Effect of the Hydrogel Carrier Structure on the Activity of Immobilized Trypsin].

The dependence of the activity of trypsin immobilized in polyacrylamide hydrogel on the hydrogel swelling ratio, the size distribution of its pores, and the means of enzyme binding has been studied. It has been shown that the most favorable conditions for immobilized trypsin are provided upon its binding to hydrogel via trypsin macromonomer copolymerization with acrylamide and a linking agent in the presence of a modifier that limits polymer chain growth.

[Novel antiproteinase hemosorbent].

A method has been developed for producing a biospecific hydrogel hemosorbent by the radical copolymerization of an unsaturated derivative of ovomucoid from duck egg white with acrylamide and N,N'-methylene bisacrylamide in an aqueous solution in the presence of mercaptoacetic acid serving as a chain transfer agent. The use of a chain transfer agent has been shown to result in changes in the structure of the hydrogel formed, namely, an increase in the degree of swelling in aqueous solutions and a decrease in the number of large pores. This creates favorable conditions for the functioning of immobilized ovomucoid and allows for an increase in the serine proteinase absorption capacity of the hemosorbent.

[Thromboresistance of glucose-containing hydrogels].

The thromboresistance of glucose-sensitive polymer hydrogels, modeling one of the functions of the pancreas, namely, the ability to secrete insulin in response to the introduction of glucose into the environment, has been studied. Hydrogels were synthesized by the copolymerization of hydroxyethyl methacrylate with N-acryloyl glucosamine in the presence of a cross-linking agent and subsequently treated with concanavalin A. Introduction of glucose residues into the hydrogel did not result in significant changes in either the number of trombocytes adhered to the hydrogel or the degree of denaturation of blood plasma proteins interacting with the hydrogel.