Evaluation of a novel point-of-care test for active matrix metalloproteinase-8: agreement between qualitative and quantitative measurements and relation to periodontal inflammation.

Background And Objectives: Severe periodontitis affects about 10% of the world population. In addition, associations between periodontitis and systemic diseases exist. Therefore, the diagnosis should be made quickly and at an early stage.

The Oral Microbiota.

The oral microbiota represents an important part of the human microbiota, and includes several hundred to several thousand diverse species. It is a normal part of the oral cavity and has an important function to protect against colonization of extrinsic bacteria which could affect systemic health. On the other hand, the most common oral diseases caries, gingivitis and periodontitis are based on microorganisms.

Confusion over live/dead stainings for the detection of vital microorganisms in oral biofilms--which stain is suitable?

Background: There is confusion over the definition of the term "viability state(s)" of microorganisms. "Viability staining" or "vital staining techniques" are used to distinguish live from dead bacteria. These stainings, first established on planctonic bacteria, may have serious shortcomings when applied to multispecies biofilms.

Matrix metalloproteinase-8 levels in peri-implant sulcus fluid adjacent to titanium and zirconium nitride surfaces.

During host interaction against oral biofilm, matrix metalloproteinase-8 (MMP-8) is activated, leading to collagenolytic destruction of host tissues. In periimplantitis patients, the active form of MMP-8 is elevated in peri-implant sulcus fluid (PISF). In this study, MMP-8 in PISF from titanium abutments and those coated with zirconium nitride (ZrN) was compared in vivo in a split-mouth design in 60 patients at 6 weeks, 6 months, and 12 months after prosthetic restoration.