Axenic cultivation of Trichomonas vaginalis in a serum-free medium.

Mammalian serum or bovine serum albumin are essential for Trichomonas vaginalis cultivated under axenic conditions. Unfortunately, these components inhibit several biological properties of these parasites. PACSR is a serum replacement, free of bovine serum albumin.

Entamoeba histolytica cysts with a defective wall formed under axenic conditions.

Axenic HK9 Entamoeba histolytica strain amoebae, maintained in PEHS medium, displayed several cystic characteristics that involve an active process of cystic wall formation, cellular volume and density diminution, and one or two nuclear divisions. The differentiation process was asynchronic, beginning after the logarithmic growth phase. The axenic cysts, which were maintained in a 50 mOsm/kg medium at 4 degrees C for 72 h, produced growing trophozoites within 1-7 days of incubation at 36 degrees C in fresh medium.

Axenic massive cultivation of Entamoeba histolytica trophozoites in spinner flasks and in a microfermentor.

A medium, PEHPS, whose main components are extracts of ox liver, and ox and swine pancreas (EHP) was used to develop two alternative methods for axenic cultivation of trophozoites in suspension: with magnetic stirrer and with a 14 l capacity microfermentor, giving the first technic yields of 2.36 x 10(5) to 3.08 x 10(5) and the second in a 14 l capacity microfermentor, which produced 1.

A multipurpose solid-phase method for protein determination with Coomassie brilliant blue G-250.

The multipurpose method for protein determination (MMPD) is based in the Coomassie brilliant blue G-250 binding to immobilized and washed proteins in paper filter disks, and the A600 measurement of the eluted protein-dye complexes. The analysis requires 5-microliters samples, put in 7-mm paper filter disks, which can be stored for up to 2 weeks, without sensible changes in their protein content. The A600 is a logarithmic function of the log of bovine serum albumin quantity, between 2.