[Chemico-toxicological study of fluoxetine].

Selected aspects of chemico-toxicological analysis of the tranquillizer drug fluoxetine are described. Optimal conditions for fluoxetin extraction from internal organs and biological fluids (blood, urine) are specified and methods proposed for its detection and quantitation including TLC, UV SPM, and HPLC. The proposed methods were verified using laboratory animals and materials for expert examination.

[The growth dynamics of nucleoli and their silver stainability in the cell cycle of ESK cells in a monolayer culture].

The cell cycle progression of individual pig embryo kidney (PK) cells was followed by time-lapse microphotography. Evidence has been presented that every detachment of cells from the substrate for subculturing leads to nearly a twofold decrease in the average number of nucleoli per nucleus. However, this number is progressively increased with every generation of flattened cells on the substrate.

[The kinetics of the transition to DNA synthesis in the cell cycle of sister cells of the ESK line in culture].

The proliferation kinetics of a cultured pig embryo kidney cell line, PK, was studied by time lapse cinemicrography and 3H-TdR autoradiography. The duration and variability of all phases of the cell cycle was estimated. Evidence is presented that the variation in the cell cycle transit time of both unrelated and sibling cells results mainly from the variation in transit of G1-phase.

[The effect of picolinic acid and iron ions on the proliferation of SPEV cells].

The effect of picolinic acid (PA) on SPEV cell proliferation is found to be different from that on normal and virus transformed NRC cells, and on spontaneously transformed CHO cells. It is shown that SPEV cells are arrested by PA at the end of G1-phase and at the beginning of S-phase and probably in G2-phase of the cell cycle. Ferrous ions remove the G1/S block induced by PA to permit the cell transfer through S-phase.