Publications by authors named "L Miranda Contreras"
Article Synopsis
- General RNA chaperones are proteins that help RNA molecules fold correctly and avoid misfolding, which is crucial for bacteria, especially with large and complex RNA structures.
- Researchers adapted a technique to screen for factors affecting RNA folding and identified eight proteins that influence the folding of a specific RNA ribozyme when deleted.
- Among these, YagL and PepA were found to bind RNA and assist in the folding process, with YagL being especially effective in promoting correct refolding of misfolded RNA, thanks to a specific protein domain responsible for its activity.
Bulk increases in nucleobase oxidation, most commonly manifesting as the guanine (G) nucleobase modification 8-oxo-7,8-dihydroguanine (8-oxoG), have been linked to several disease pathologies. Elucidating the effects of RNA oxidation on cellular homoeostasis is limited by a lack of effective tools for detecting specific regions modified with 8-oxoG. Building on a previously published method for studying 8-oxoG in DNA, we developed ChLoRox-Seq, which works by covalently functionalizing 8-oxoG sites in RNA with biotin.
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- RNA oxidation, particularly the formation of 8-oxo-7,8-dihydroguanosine (8-oxo-rG), serves as a key indicator of oxidative stress in cells.
- Polynucleotide phosphorylase (PNPase) helps protect cells from oxidative stress by recognizing and degrading RNA containing 8-oxo-rG, but how 8-oxo-rG affects this process was previously unclear.
- This study finds that 8-oxo-rG causes PNPase to stall during RNA degradation, particularly due to a crucial residue (Arg399), influencing bacterial survival under stress conditions.
Stricturing Crohn's disease and intestinal endometriosis: An unusual coexistence.
Rev Gastroenterol Mex (Engl Ed)
October 2024