Oncolytic virus purification with periodic counter-current chromatography.

Virus-based biologicals are one of the most promising biopharmaceuticals of the 21st century medicine and play a significant role in the development of innovative therapeutic, prophylactic, and clinical applications. Oncolytic virus manufacturing scale can range from 5 L in research and development up to 50 L for clinical studies and reach hundreds of liters for commercial scale. The inherent productivity and high integration potential of periodic counter-current chromatography (PCC) offer a transversal solution to decrease equipment footprint and the reduction of several non-value-added unit operations.

Definition and dynamic control of a continuous chromatography process independent of cell culture titer and impurities.

Advances in cell culture technology have enabled the production of antibody titers upwards of 30g/L. These highly productive cell culture systems can potentially lead to productivity bottlenecks in downstream purification due to lower column loadings, especially in the primary capture chromatography step. Alternative chromatography solutions to help remedy this bottleneck include the utilization of continuous processing systems such as periodic counter-current chromatography (PCC).

Filter-based treatment of leachate from an industrial landfill containing shredder residues of end-of-life vehicles and white goods.

A pilot plant was set up to treat leachate from an industrial landfill containing shredder residues of end-of-life vehicles and white goods. The treatment plant consisted of aeration and sedimentation steps for pre-treatment, and a filter. The plant was designed to simultaneously remove various types of pollutants.

Hydraulic performance of a full-scale peat and ash biofilter in treatment of industrial landfill leachate.

The purpose of this study was to evaluate the hydraulic performance of a full-scale on-site vertical-flow biofilter, consisting of a mixture of peat and carbon-containing ash, and a 500 m(3) equalization pond prior to the filter-system. The treatment plant was constructed to clean up leachate from an industrial mono-landfill that contained shredder residues of end-of-life vehicles and white goods. With the limited storage capacity of the equalization pond, peak loading rates exceeded up to five to six times the designed daily hydraulic load limit of the biofilter system.

Performance of a full-scale biofilter with peat and ash as a medium for treating industrial landfill leachate: a 3-year study of pollutant removal efficiency.

Shredder residues of end-of-life vehicles and white goods are a complex waste stream, which nowadays most often is disposed of at industrial landfills. This paper describes the most important findings concerning the complex composition of the landfill leachate and its on-site, year-round treatment under cold-climate conditions. A 3-year investigation has confirmed that concentrations of different types of pollutants, most of them at low initial concentrations, can be simultaneously reduced in vertical-flow biofilters consisting of a mixture of peat and carbon-containing ash.