Publications by authors named "L Manso-Silvan"
We investigated the interactions of unopsonized and opsonized Mycoplasma mycoides subsp. mycoides (Mmm) with bovine macrophages in vitro. Mmm survived and proliferated extracellularly on bovine macrophage cell layers in the absence of Mmm-specific antisera.
View Article and Find Full Text PDFVaccination is the most cost-effective tool to control contagious bovine pleuropneumonia. The vaccines currently used in Africa are derived from a live strain called T1, which was attenuated by passage in embryonated eggs and broth culture. The number of passages is directly correlated to the degree of attenuation of the vaccinal strains and inversely correlated to their immunogenicity in cattle.
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- The subspecies mentioned causes contagious caprine pleuropneumonia (CCPP), a serious goat disease that affects livestock production in Africa and Asia and is recognized as a notifiable disease by the World Organisation for Animal Health (WOAH).
- Current vaccines are inadequate according to WOAH standards and there are concerns about their effectiveness, largely due to a lack of genome engineering tools for studying the disease.
- Recent advancements in synthetic biology allowed researchers to create targeted genetic mutations in the pathogen, leading to the development of improved methods for identifying virulence factors and designing better vaccines against CCPP.
The genetic engineering of genome fragments larger than 100 kbp is challenging and requires both specific methods and cloning hosts. The yeast is considered as a host of choice for cloning and engineering whole or partial genomes from viruses, bacteria, and algae. Several methods are now available to perform these manipulations, each with its own limitations.
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