How molting of laying hens influences body composition and blood parameters.

The physiological and metabolic changes laying hens undergo during molt are poorly understood, but could aid in understanding why hens stop egg production during the first cycle of lay. We therefore induced a molt and studied how this influenced body composition, blood parameters and production performance. Additionally, four diets postmolt were fed in a 2 × 2 factorial design with two levels of metabolisable energy lay (ME Lay; low = 11.

Sisonke Ntwana: Describing the mechanisms of change of an arts-based intervention for pregnant and parenting adolescents experiencing violence and poor mental health in South Africa.

Background: In South Africa, one in five adolescents experience pregnancy and face heightened rates of interpersonal violence and mental health challenges. Yet, few interventions are tailored to them.

Methods: 28 pregnant adolescents reporting past year intimate partner violence and/or non-partner rape were purposively recruited in antenatal clinics in Johannesburg to attend a 6-session arts-based intervention, delivered by 4 graduate art therapy students alongside clinical supervision.

The Dutch Citizen's Understanding and Perception of the Actors Involved in the Netherlands' COVID-19 Pandemic Response: A Focus Group Study During the First Pandemic Wave.

Introduction: The COVID-19 pandemic was a public health emergency (PHE) of unprecedented magnitude and impact. It provided the possibility to investigate the Dutch citizens' understanding and perception of the actors involved in the Dutch pandemic response as a PHE unfolded.

Methods: Three focus groups (FGs) were held with 16 Dutch citizens in June 2020.

The influence of breed, dietary energy and lysine on laying persistency and body composition of laying hens.

The effect of dietary energy and lysine levels on laying persistency and body composition in brown and white hens was studied. Dietary treatments with 2 Metabolizable Energy levels (ME lay; constant or reduction over time) and 2 apparent fecal digestible Lys levels (AFD Lys; constant or reduction over time), were fed to Lohmann white or brown hens, from 17 to 75 wk of age, in a 2 × 2 × 2 factorial design. Data were subjected to mixed model analyses.

A Photocrosslinking Probe to Capture the Substrates of Caseinolytic Protease P.

Protein homeostasis in bacteria is regulated by proteases such as the tetradecameric caseinolytic protease P (ClpP). Although substrates of ClpP have been successfully deciphered in genetically engineered cells, methods which directly trap processed proteins within native cells remain elusive. Here, we introduce an in situ trapping strategy which utilizes trifunctional probes that bind to the active site serine of ClpP and capture adjacent substrates with an attached photocrosslinking moiety.