DNA Methylation, Deamination, and Translesion Synthesis Combine to Generate Footprint Mutations in Cancer Driver Genes in B-Cell Derived Lymphomas and Other Cancers.

Cancer genomes harbor numerous genomic alterations and many cancers accumulate thousands of nucleotide sequence variations. A prominent fraction of these mutations arises as a consequence of the off-target activity of DNA/RNA editing cytosine deaminases followed by the replication/repair of edited sites by DNA polymerases (pol), as deduced from the analysis of the DNA sequence context of mutations in different tumor tissues. We have used the weight matrix (sequence profile) approach to analyze mutagenesis due to Activation Induced Deaminase (AID) and two error-prone DNA polymerases.

[Influence of isoniazid complex with A-I apolipoprotein on activity of lysosomal enzymes in mice with tuberculous inflammation model].

It is established that isoniazid (isonicotinic acid hydrazide) can interact with A-I apolipoprotein to form a complex, which can be considered as the transport form of the preparation. The use of this complex for the treatment of mice with BCG-induced tuberculous inflammation led to an increase in the free activities of acid phosphatase and cathepsin D in the liver, which was decreased under the action of mycobacteria and the free form of isoniazid. The isoniazid complex with A-I apolipoprotein exhibited more expressed anti-inflammatory effect (estimated by the activity of chitotriosidase in blood serum) as compared to the free drug.

[High-density lipoproteins as a form of daunorubicin transport in hepatoma cells of mice].

The efficiency of using high-density lipoproteins (HDLPs) as the transport form of an antineoplastic drug daunorubicin (rubomycin hydrochloride, daunoxome) has been shown on the culture of HA-1 hepatoma cells of mice. The use of HDLPs in a complex with daunorubicin led to an increase in the efficiency of drug transport and cytotoxic action with respect to tumor cells in comparison with hepatocytes of healthy animals.

[The analysis of interaction between lipoproteins and steroid hormones].

Using the methods of ultracentrifugation, gel-filtration and fluorescence quenching, we demonstrated, that plasma lipoproteins bind steroid hormones and can therefore play a role of their active transport form in an organism. High density lipoproteins have revealed the highest affinity to steroids for. It has been found, that protein component of lipoproteins takes part in the formation of lipoprotein-steroid complex.

[Role of tumor-associated macrophages in the regulation of protein biosynthesis in Ehrlich carcinoma cells].

The paper discusses the role of peritoneal macrophages in uptake and metabolic degradation of high-density proteins of lipoproteins in mice with Ehrlich ascites carcinoma. These processes were found to be influenced by cortisol. Distinctions in spectra of endocellular proteins in tumor-associated macrophages and peritoneal ones in intact mice were identified and, in particular, relative to apolipoprotein E level.