[Antimicrobial activity of Laetiporus sulphureus strains grown in submerged culture].

Cultural conditions for growth and fruit body formation were elaborated to four strains of Laetiporus sulphureus isolated from nature. All strains demonstrated antimicrobial activity against a wide spectrum of gram-positive and gram-negative bacteria during agar and submerged cultivation including methicillin-resistant strain of Staphylococcus aureus (MRSA) and glycopeptide-resistant strain of Leuconostoc mesenteroides. Antifungal activity was not found.

[Effect of penicillin precursors on antibiotic biosynthesis in various strains].

The regularities of biosynthesis of 6-aminopenicillanic acid (6-APA), benzylpenicillin (BP) and phenoxymethylpenicillin (PMP) by the strains under the investigation did not significantly differ. In the absence of the precursor both the strains mainly synthesized 6-APA. Phenylacetic acid (PAA) and phenoxyacetic acid (POAA) provided directed biosynthesis: the fungus synthesized BP or PMP depending on the precursor nature.

[Effect of the methods of sterilization on the quality of nutrient media and on the level of antibiotic production].

It was shown that conditions for heat sterilization of nutrient media for biosynthesis of antibiotics had an impact on their biochemical composition and activity of a fermentation broth in production of penicillin and erythromycin. The temperature of 120 degrees C and the time of 25 minutes proved to be optimal for sterilization of the media in regard to both preservation of their biochemical composition and providing of the maximum antibiotic productivity on the one hand and maintenance of the sterility during the fermentation process on the other hand.

[Ampicillin pharmacokinetics in prostatic adenoma patients].

Studies on ampicillin pharmacokinetics in patients with prostate adenoma showed that adenoma did not influence the antibiotic kinetics. The ampicillin maximum concentration in tissues and organs of the urinary corresponded to the peak concentrations in blood. Therefore, development of dosage regimens providing the drug efficient concentrations in tissues and organs of the urinary tract should be based on ampicillin kinetics in the blood.