Investigation of the effects of deracoxib and piroxicam on the in vitro viability of osteosarcoma cells from dogs.

Objective: To determine whether exposure of canine osteosarcoma cells to deracoxib or piroxicam results in decreased viability, whether the cytotoxic effects of deracoxib and piroxicam involve induction of apoptosis, and whether deracoxib is a more potent inhibitor of osteosarcoma cell growth than piroxicam.

Sample Population: 1 fibroblast and 3 osteosarcoma cell lines.

Procedure: Cell counts and viability assays were performed using osteosarcoma cells (POS, highly metastatic POS, and canine osteosarcoma cell 31) and fibroblasts after 72 hours of incubation with deracoxib at concentrations of 0.

Investigation of the effect of pamidronate disodium on the in vitro viability of osteosarcoma cells from dogs.

Objective: To determine the effect of pamidronate disodium on the in vitro viability of osteosarcoma cells and non-neoplastic cells from dogs.

Sample Population: 3 osteosarcoma and 1 fibroblast cell lines derived from dogs.

Procedure: Cell counts and cell viability assays were performed in cultures of osteosarcoma cells (POS, HMPOS, and COS31 cell lines) and fibroblasts after 24, 48, and 72 hours of incubation with pamidronate at concentrations of 0.

The effect of topical tripeptide-copper complex on healing of ischemic open wounds.

Objective: To evaluate the effects of topical glycyl-L-histidyl-L-lysine tripeptide-copper complex (TCC; Iamin 2% Gel; Procyte Corporation, Redmond, WA) on healing in ischemic open wounds.

Study Design: Experimental study.

Sample Population: Twenty-four adult male Sprague-Dawley rats.

Malabsorption of zinc in rats with acetic acid-induced enteritis and colitis.

Acute intestinal inflammation was established in rats by intraluminal administration of acetic acid into loops of distal ileum, proximal jejunum or ascending colon. The study included two control groups of intact (untreated) rats and sham-operated (saline-treated) rats for each intestinal segment. A third group of rats received acetic acid.

Regulation of cysteine-rich intestinal protein by dexamethasone in the neonatal rat.

The cysteine-rich intestinal protein (CRIP) is an intestinal zinc-binding protein containing a single copy of a cysteine-rich domain known as the LIM motif. CRIP mRNA and protein levels increased in the rat small intestine throughout the suckling period, reaching highest levels by the late weanling stage. A similar developmental pattern of CRIP protein levels was also detected by an increase in zinc binding to CRIP-containing HPLC fractions of intestinal cytosol.