Publications by authors named "L M Krauss"
Background: Detailed reports are scarce on minimally-invasive tracheostomy (MIT) techniques for critically ill patients with challenging anatomy or complex coagulopathies. In such high-risk patients, conventional percutaneous dilatational tracheostomy (PDT) may lead to severe complications.
Methods: Aiming to broaden the scope of MIT for patients previously excluded due to high risks, we developed a new care bundle (MIT technique), specifically designed for intensive care specialists.
Golden Gate assembly (GGA) can seamlessly generate full-length genes from DNA fragments. In principle, GGA could be used to design combinatorial mutation libraries for protein engineering, but creating accurate, complex, and cost-effective libraries has been challenging. We present GGAssembler, a graph-theoretical method for economical design of DNA fragments that assemble a combinatorial library that encodes any desired diversity.
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- * Direct KRAS inhibitors are showing promise in clinical trials, but resistance to treatment is a concern, prompting the search for combination therapies.
- * Unbiased drug screening identified effective combinations involving SOS1 inhibitors, PTPN11/SHP2 inhibitors, and multi-kinase inhibitors, validated using a unique KRAS-mutated patient-derived organoid model.
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- Cancer cells adapt to various stresses, including those from treatments, through metabolic adaptability, focusing on the energy sensor AMP-activated protein kinase (AMPK).
- In pancreatic ductal adenocarcinoma (PDAC), high levels of AMPK expression and activity were observed, leading to the identification of PF-3758309 as a potential AMPK inhibitor through drug repurposing.
- PF-3758309 not only demonstrates pre-clinical effectiveness in PDAC models but also helps sensitizes cancer cells to ferroptosis inducers, paving the way for AMPK-targeted therapies in combination treatments for this type of cancer.
Article Synopsis
- Dealing with different sequence formats and reference genomes is difficult in genetic research, leading to the need for tools that simplify this process.
- The Sequence Conversion and Analysis Toolbox (SeqCAT) has been created to help researchers standardize and convert gene variant coordinates efficiently through a user-friendly web interface and API for automation.
- With features like converting protein positions to DNA and checking gene fusions, SeqCAT offers 14 applications to cover a variety of genetic research needs and is accessible online.