Potential of nanopore sequencing for tuberculosis diagnosis and drug resistance detection.

Objectives: This study evaluates the effectiveness of nanopore sequencing for accurate detection of Mycobacterium tuberculosis pathogens and drug resistance mutations in clinical specimens.

Methods: A retrospective analysis of 2,421 specimens from suspected tuberculosis patients admitted to Xi'an Chest Hospital from 2022 to 2023 was conducted, with 131 specimens undergoing via real-time, fluorescence-based quantitative Polymerase Chain Reaction (qPCR), simultaneous amplification and testing RNA (RNA), Mycobacterium culture, Mycobacterium smear, and nanopore sequencing. Employing clinical tuberculosis diagnoses as the gold standard, sensitivity, specificity, positive predictive value, negative predictive value, concordance rate, and Kappa coefficient were measured for the five detection techniques.

Highly Selective Construction of Unique Cyclic [4]catenanes ( 841 ) Induced by Multiple Noncovalent Interactions.

The synthesis of high-ordered mechanically interlocked supramolecular structures is an extremely challenging topic. Only two linear [4]catenanes have been reported so far and there is no defined strategy to obtain cyclic [4]catenane. Herein, two unprecedented cyclic [4]catenanes, 1 and 2, were prepared in high yields.

Effects of Probucol on plasma amyloid-β transport in patients with hyperlipidemia: a 12-week randomized, double-blind, placebo-controlled trial.

Background: Although dyslipidemia has been acknowledged as a risk factor for Alzheimer's disease (AD), the effects of lipid-lowering drugs on AD have not been determined. The primary pathophysiological hallmark of AD is the deposition of amyloid-β (Aβ) plaques in the brain. Plasma Aβ levels are influenced by the transport of Aβ from the central nervous system to the peripheral blood.

New record and dietary ecology of a poorly-known treefrog, Yu, Hui, Hou, Wu, Rao & Yang, 2019 (Anura, Rhacophoridae) in Vietnam.

Background: The White-lipped Treefrog Yu, Hui, Hou, Wu, Rao & Yang, 2019 was previously confused with (Blyth, 1852). Records of from Yunnan (China), Vietnam and Thailand were recognised as a misidentification and this taxon was subsequently described as a new species, based on morphological and molecular evidence. This species is currently known from southern China, Thailand, Laos and Vietnam.