Cell wall glycopolymers of Streptomyces albus, Streptomyces albidoflavus and Streptomyces pathocidini.

The cell wall glycopolymers of three strains of Streptomyces albus and the type strain of Streptomyces pathocidini were investigated. The structures of the glycopolymers were established using a combination of chemical and NMR spectroscopic methods. The cell wall of S.

Identification of Streptomyces coelicolor M145 genomic region involved in biosynthesis of teichulosonic acid-cell wall glycopolymer.

The cell wall of the model actinomycete Streptomyces coelicolor M145 has recently been shown to contain the novel glycopolymer teichulosonic acid. The major building block of this polymer is 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (Kdn), suggesting initial clues about the genetic control of biosynthesis of this cell wall component. Here, through genome mining and gene knockouts, we demonstrate that the sco4879-sco4882 genomic region of S.

Novel teichulosonic acid from cell wall of Streptomyces coelicolor M145.

The cell wall of Streptomyces coelicolor M145, a prototrophic plasmidless (SCP1(-) SCP2(-)) variant of strain S. coelicolor A3(2) contains the main glycopolymer represented by Kdn-containing teichulosonic acid with unusual structure which has not been described so far: [structure: see text]. The minor polymer was found to be a poly(diglycosyl 1-phosphate) with the following repeating unit: -6)-α-Galp-(1→6)-α-GlcpNAc-(1-P-.

Novel teichulosonic acid from cell walls of some representatives of the genus Kribbella.

Cell walls of each of five bacterial strains belonging to the genus Kribbella (family Nocardioidaceae, order Actinomycetales) contain a neutral polysaccharide (mannan) and teichulosonic acid of novel structure in different proportions. The novel teichulosonic acid found in strains VKM Ac-2500, VKM Ас-2568, VKM Ас-2572, and VKM Ас-2575 is a heteropolymer with an irregular structure where fragments I (predominant) alternate with fragments II (minor): The teichulosonic acid from Kribbella sp. VKM Ac-2527 has in general a structure similar to that above with the exception that the Pse residue is randomly glycosylated at O-4 with β-l-Rhap (along with α-d-Galp3OMe or α-d-Galp2,3OMe).

[Diaskintest for tuberculosis in children and adolescents].

To study whether Diaskintest might be used as a criterion for the activity of local tuberculous changes in children and adolescents, the authors examined three groups of patients aged 0 to 17 years: 1) 20 patients with new-onset tuberculosis in the progression phase; 2) 25 patients with new-onset tuberculosis in the regression phase with the signs of consolidation and calcification; 3) 18 tuberculosis dispensary Group IIIB patients who received the basic course of therapy and had residual posttuberculous changes as calcinates and compact foci. Group 3 was found to greatly differ from Groups 1 and 2 in the lower rates of positive reactions, the higher rate of negative reactions, and smaller papulae while the Mantoux test with 2 TE indicated that in Group 3, the papulae were smaller than those in Group 2. Responses to the Diaskintest test versus the Mantoux 2TE test were significantly more pronounced in patients with progressive tuberculosis and less marked in dispensary Group IIB patients who received the basic course of antituberculous therapy.