Western blot analysis of cereal grain prolamins using an antibody to carboxyl-linked indoleacetic Acid.

A monoclonal antibody raised against carboxyl-linked IAA was used in Western blot analysis of storage proteins from kernels of Avena sativa, Pennisetum americanum, Sorghum bicolor, and Zea mays. IAA or an IAA-like molecule is associated with the ethanolsoluble protein fraction of the seed. Western blotting of commercial zein, the major storage protein of maize, along with physicochemical evidence reported by Leverone et al.

Evidence of zein-bound indoleacetic Acid using gas chromatography-selected ion monitoring-mass spectrometry analysis and immunogold labeling.

Commercial zein was base-hydrolyzed and purified extracts were subjected to gas chromatography-selected ion monitoring-mass spectrometry analysis. Indoleacetic acid (IAA) was shown to be released from this storage protein of corn (Zea mays). Isotope dilution using [(13)C(6)]IAA as an internal standard revealed a conservative ratio of 1 mole IAA to 175 moles zein.

Enhancement of interleukin-1 activity by murine cytomegalovirus infection of a macrophage cell line.

The effect of murine cytomegalovirus (MCMV) infection on interleukin 1 (IL-1) secretion was assessed using the macrophage cell lines P388D1 and J774A.1. The former proved to be nonpermissive for MCMV in that infectious virus and viral immediate early protein (pp89) were not expressed in these cells.