Publications by authors named "L L Mariani"
Background: Interstitial fibrosis and tubular atrophy (IFTA), and density and shape of peritubular capillaries (PTCs), are independently prognostic of disease progression. This study aimed to identify novel digital biomarkers of disease progression and assess the clinical relevance of the interplay between a variety of PTC characteristics and their microenvironment in glomerular diseases.
Methods: A total of 344 NEPTUNE/CureGN participants were included: 112 minimal change disease, 134 focal segmental glomerulosclerosis, 61 membranous nephropathy, and 37 IgA nephropathy.
In preclinical experiments, cyclic fasting-mimicking diets (FMDs) showed broad anticancer effects in combination with chemotherapy. Among different tumor types, triple-negative breast cancer (TNBC) is exquisitely sensitive to FMD. However, the antitumor activity and efficacy of cyclic FMD in TNBC patients remain unclear.
View Article and Find Full Text PDFReusing test cases across apps that share similar functionalities reduces both the effort required to produce useful test cases and the time to offer reliable apps to the market. The main approaches to reuse test cases across apps combine different semantic matching and test generation algorithms to migrate test cases across Android apps. In this paper we define a general framework to evaluate the impact and effectiveness of different choices of semantic matching with Test Reuse approaches on migrating test cases across Android apps.
View Article and Find Full Text PDFA significant challenge for chimeric antigen receptor (CAR) T cell therapy against glioblastoma (GBM) is its immunosuppressive microenvironment, which is densely populated by protumoral glioma-associated microglia and macrophages (GAMs). Myeloid immune checkpoint therapy targeting the CD47-signal regulatory protein alpha (SIRPα) axis induces GAM phagocytic function, but CD47 blockade monotherapy is associated with toxicity and low bioavailability in solid tumors. In this work, we engineer a CAR T cell against epidermal growth factor receptor variant III (EGFRvIII), constitutively secreting a signal regulatory protein gamma-related protein (SGRP) with high affinity to CD47.
View Article and Find Full Text PDFArticle Synopsis
- - Cell fate is influenced by specific transcription factors called 'pioneer' factors that can bind to target sites within nucleosomes to initiate chromatin opening, yet they only bind a small fraction of their potential binding sites in the genome.
- - Researchers developed a method called PIONEAR-seq to study how seven human pioneer TFs bind to nucleosomes, revealing that the binding preferences depend more on the local sequence context than on the properties of the TFs themselves.
- - The study suggests that the physical characteristics of nucleosomal DNA, such as its flexibility and rigidity, play a role in determining the binding locations of pioneer factors within nucleosomes, adding a new layer of regulatory information for transcription factors in eukaryotic cells.