Publications by authors named "L L Lienou"

Carvacrol (CHO), an efficient phenolic antioxidant substance for several cell types, may become a useful antioxidant for female germ cells and embryo culture. This study investigates the effects of carvacrol supplementation on bovine oocytes in maturation (IVM) and embryo production. In total, 1222 cumulus-oocyte complexes were cultured in TCM-199 alone (control treatment) or supplemented with carvacrol at the concentrations of 3 µM (Carv-3), 12.

View Article and Find Full Text PDF

This study investigates the impact of eugenol (EU) supplementation on bovine oocyte in vitro maturation (IVM) and antioxidant capacity, as well as in vitro embryo production and quality after conventional in vitro fertilization (IVF). A total of 1077 cumulus oocyte complexes were cultured in TCM-199 without EU supplementation (control treatment) or supplemented with EU at the concentrations of 10 μM (EU-10), 20 μM (EU-20), or 40 μM (EU-40). After IVM, the oocytes were subjected to IVF and embryo culture.

View Article and Find Full Text PDF

Dicliptera verticillata is a medicinal plant traditionally used in western Cameroon to cure female infertility. This experiment was designed to assess the effects of the aqueous extract of Dicliptera verticillata (AEDv) on fertility and gestation in female rats. Oral increasing doses of AEDv were administered to immature female rats over 20 d.

View Article and Find Full Text PDF

The present study evaluated the effect of the aqueous extract from leaves of E. speciosa on some physiological and biochemical parameters of reproduction and the onset of puberty in pregnant mare serum gonadotropin (PMSG)-primed immature female rats. High pressure liquid chromatography (HPLC) was used to quantify the phenolic compounds in the methanol/methylene chloride (1:1) extract, the ethanolic and ethyl acetate fractions and the aqueous residue of E.

View Article and Find Full Text PDF

Background: Senecio biafrae is a medicinal plant widely used in traditional medicine to cure female infertility. Some effects have been pharmacologically demonstrated on immature female rats but in vivo and in vitro investigations are still necessary for determining its mechanism of action. The aim of the present study was to evaluate the estrogenic and FSH-like effects of the plant extracts and fractions on some fertility parameters in immature female rats and on in vitro survival and growth of swine preantral follicles.

View Article and Find Full Text PDF