Maternal incompatibilities with fetal human platelet alloantigens -1a, -1b and -15 are the main causes of neonatal alloimmune thrombocytopenia in Russia.

Aim: Mechanisms underlying the development of neonatal alloimmune thrombocytopenia (NAIT) in in Russia have been studied.

Materials And Methods: Genetic polymorphisms of human platelet alloantigens (HPA) -1, -2, -3, -4, -5, and -15 were evaluated in 27 families having the newborns with NAIT. NAIT was diagnosed according to the following criteria: (1) newborn with thrombocytopenia; (2) mother with no thrombocytopenia and no increase of platelet associated IgG, (3) presence of antibodies reacting with paternal platelets in maternal plasma / serum.

[Molecular serological characteristics of weak D antigen types of the Rhesus system].

Aim: to estimate the spread of weak D antigen types of the Rhesus system in the citizens of the Russian Federation and a possibility of serologically identifying these types.

Subjects And Methods: The red blood cells and DNA of people with weakened expression of D antigen were investigated using erythrocyte agglutination reaction in salt medium (2 methods); agglutination reaction in the gel columns containing IgM + IgG anti-D antibodies, indirect antiglobulin test with IgG anti-D antibodies (2 methods); polymerase chain reaction to establish the type of weak D.

Results: A rhesus phenotype was determined in 5100 people in 2014-2015.

[Determinants for iron overload in patients with acute leukemias and aplastic anemia].

Aim: to reveal the determinants of the development of iron overload in patients with acute leukemias (AL) and aplastic anemia (AA).

Subjects And Methods: The investigation included 104 patients, including 64 with various types of AL, 31 with AA, and 9 with myelodysplastic syndromes (MDS). A group affiliation and an erythrocyte phenotype were determined from rhesus system antigens in all the patients and the HFE gene was studied to identify mutations.

Genetic differences by platelet-specific antigens used for monitoring allomyelotransplant engraftment.

Monitoring of allomyelotransplant engraftment is essential for evaluation of the function of transplanted cells and timely diagnosis of disease relapse. The possibility of using differences by HPA genes for monitoring of survival of transplanted hemopoietic stem cells was studied. Differences by HPA genes in HLA-identical sibs can be used for the analysis of chimerism after myelotransplantation.