[Chemiluminescent determination of iron, copper and cobalt in the serum].

Iron in the presence of diethylenetriamine, an activator, is a catalyst of the chemiluminescent reaction between 4-diethylaminophthalhydraside and hydrogen peroxide. The following conditions are optimal for iron content determination: concentration of 4-diethylaminophthalhydraside - (4-5) X 10(-5) M, H2O2 - 1 X 10(-3) M, diethylenetriamine - 5 X 10(-4) M; pH 10-11. Chemoluminescent reaction between 4-diethylaminophthalhydrazide and hydrogen peroxide is catalyzed also by copper and cobalt ions.

[Chemiluminescent determination of copper in the serum].

A procedure for chemiluminescent determination of copper in the blood serum ash is suggested using an indicator reaction between 4-aminophthalhydrazide and hydrogen peroxide permitting copper to be determined in a minimum (0.003 micrograms) concentrations in 1 ml of the blood serum.

[Isolation and study of the properties of the hemagglutinins produced by Streptomyces badius actinomycetes].

Hemagglutinin inducing polyagglutination of erythrocytes in the presence of the group blood sera was isolated from the supernatant of the culture fluid of Streptomyces badius 0626 by precipitation with ammonium sulfate and subsequent chromatography on Sephadex. Hemagglutinin proved to be a protein containing no lipid or carbohydrate components and possessing no bacterioagglutinating ability. Examination of the culture fluid supernatants of 150 cultures did not reveal any strains definitely exhibiting the transformation capability for human blood groups.