Publications by authors named "L Kh Pastushkova"
Background/objectives: The development of blood tests for the early detection of individual predisposition to socially significant diseases remains a pressing issue.
Methods: In this pilot study, multiple reaction monitoring mass spectrometry (MRM-MS) with a BAK-270 assay was applied for protein concentrations analysis in blood plasma from 21 healthy volunteers of the European cohort.
Results: The levels of 138 plasma proteins were reliably and precisely quantified in no less than 50% of samples.
The paper presents an analysis of the proteomic composition in relation to both the risk of thrombosis and changes in the state of cardiomyocytes associated with the risk of cardiac fibrosis and heart failure. We examined 12 practically healthy male volunteers exposed to head-down -6° tilt bed rest (HDBR) for 21 days. The revealed decrease in the level of stimulating growth factor 2 (ST2) on days 10 and 21 relative to the initial values (background; 5 days before HDBR) indicated a decrease in the myocardial load and cardiomyocyte extensibility.
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- Head-down bed rest (HDBR) simulates the effects of weightlessness on the human body, particularly focusing on how it impacts the cardiovascular system over a 21-day period.
- *The study found that HDBR increased levels of various proteins related to cardiovascular diseases and processes like blood clotting and inflammation, suggesting negative effects on heart health.
- *Conversely, some proteins involved in essential functions like oxygen transport and cell structure decreased during the HDBR, indicating overall physiological stress on the body.
In order to identify changes in the blood proteome of healthy volunteers after passive tilt test carried out on day 19 of head-down bed rest, a chromato-mass-spectrometric analysis of samples of dried blood spots was carried out. It was revealed that the body's response to the tilt test was characterized by a decrease in the level of HDL and kininogen-1. After the tilt test, we observed an increase in the level of vimentin, vitamin K-dependent protein C, Wnt signaling pathway proteins, proteins involved in autophagy and adaptive immune response, focal adhesion proteins, vascular damage marker S100A8, PEDF regulator, and some proteins of the heart: cardiac actin ACTC1 and transcription factor GATA4.
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