Is pneumatosis cystoides intestinalis gas-distended and ruptured lymphatics? Reappraisal by immunohistochemistry.

Context: Pneumatosis cystoides intestinalis (PCI) is a condition with multiple gas-filled cysts within the bowel wall, associated with diverse background diseases. Its pathogenesis is still a mystery. Some previous observations scattered in the literature have suggested an association of the cystic spaces in PCI with the lymphatics.

[Multidrug resistance increase of tumor cells at the effect of radiation and phorbol ether depends on protein kinase C and reactive oxygen species].

The effects of phorbol ether (PMA) and ionizing radiation on multidrug resistance (MDR) of human larynx cancer cells HEp-2 and the dependences of these effects on protein kinase C (PKC) activity and reactive oxygen species (ROS) production were studied. MDR was determined by transport rate of rhodamine 123 off cells and production of ROS in cells was measured by means of 2'7'-dichlorodigidrofuorescein oxidation to fluorescent 2',7'-dichlorofluorescein. ROS production was increased in cells at PMA treatment.

[On the biophysical mechanism of the circum-hour cell rhythm and its role in metabolism].

The universal biophysical mechanism of "circum-hour" oscillations of parameters and properties of various cells and their organelles was considered. The mechanism is the result of nonspecific responses of cells to any damaging agents. At the basis of the "circum-hour" mechanism is the earlier reported inverse dependence of the activity of enzymes on the concentration of low-molecular weight organic substances in medium.

[On the mechanism of the nonspecific cell response to the action of damaging agents and the nature of hormesis].

A conception of the molecular mechanism of the universal nonspecific cell response (NCR) to the action of all damaging factors of physical and chemical nature is described. The mechanism is shown to be closely related to the general principles of the structural-functional organization of cells and to the properties of biological macromolecules. Among them are the phenomenon of nonspecific regulation of the enzyme activity by low-molecular substances and the compartmentation of the latter within the cell, which is provided by the system of intracellular membranes.

[Interphase death of irradiated thymocytes--result of the "bystander effect"].

It was shown that interphase death of thymocytes, which mechanism was established by author's laboratory already over 10 years ago belongs to phenomena which recently was named as caused by "bystander effect".