Ethanol-induced structural and functional alterations of epidermal growth factor receptor in buccal mucosa.

1. Ethanol treatment caused a 47% decrease in [125I]EGF binding to the membrane preparations of buccal mucosa resulting from the decrease of binding sites rather than the affinity of high affinity receptor. 2.

Effect of ethanol on epidermal growth factor receptor in buccal mucosa.

The effect of ethanol on buccal mucosal epidermal growth factor receptors was investigated. The buccal mucosal cells from adult rats were incubated in DMEM at 4 degrees C and 37 degrees C in the presence of various concentrations (0-5%) of ethanol and then assayed for EGF receptor binding using [125I]-EGF. The results of binding analysis showed that the cells incubated in the presence of ethanol displayed diminished [125I]-EGF binding.