Chitin and Products of Its Hydrolysis in Vibrio cholerae Ecology.

The role of chitin and its hydrolysis products generated by Vibrio cholerae chitinases in mechanisms of its adaptation in water environments, metabolism, preservation, acquisition of pathogenic potential, and its epidemiological value are reviewed. Chitin utilization by V. cholerae as a source of energy, carbon, and nitrogen is described.

[Cholera Vibrio biofilm: production, characterization and role in reservation of causative agent in water environment].

Aim: Experimental production, characterization and evaluation of the role of cholera vibrio biofilm.

Materials And Methods: 33 strains of Vibrio cholerae eltor O1 and V. cholerae O139 of various epidemic significance and origin were studied in a series of experiments by bacteriologic, microscopic (light-optic, luminescent, scanning electron microscopy), molecular genetics, spectrophotometric and statistical methods.

Whole-Genome Sequencing of a Vibrio cholerae El Tor Strain Isolated in the Imported Cholera Focus in Siberia.

The draft genome sequence of Vibrio cholerae O1 strain I-1263, isolated from a patient in the imported focus in Siberia, was determined. The established structural features of the mobile genetic elements indicate stage-by-stage formation of a highly pathogenic V. cholerae clone and promote understanding of the mechanisms of evolutionary pathogen transformations.

[MALDI-TOF mass-spectrometric analysis in the accelerated identification of the Vibrio genus microorganisms].

The goal of this work was to develop methodological approaches to identification of the Vibrio genus representatives using the MALDI-TOF mass-spectrometric analysis technologies. The aspects of the biological safety in sample preparations for mass-spectrometric analysis were studied, reference spectra of six typical V. cholerae strains were developed.

[Membrane-bound proteases of ompT+ and ompT- Vibrio cholerae strains].

Aim: Detection ofproteases in outer membranes (OM) of ompT+ and ompT- Vibrio cholerae strains of O1 and O139 serogroups.

Materials And Methods: Specific sterile preparations of OM were obtained by lysis of live V. cholerae cells by 4.