[Hemolytic activity of new hybrid antioxidants ichfans].

The hemolytic activity of new spatially screened hybrid antioxidants--ichfans--has been studied. The concentration-dependent hemolytic action of high concentrations of ichfans with 8, 10, 12 or 16 carbon atoms in the aliphatic chain has been revealed. A nonmonotonic dependence of the hemolytic activity on the hydrophobic properties of ichfans has been found.

[Effect of ichphans on the structural properties of erythrocyte membranes].

Morphological transformation of erythrocytes and structural changes in the erythrocyte membrane have been revealed by scanning electron microscopy and spin-probe technique. These effects were caused by the incorporation of ichphans, new generation drugs combining antioxidant and anticholinesterase effects, into the erythrocyte membrane and their distribution in the intramembrane space. Different distribution and modulatory effect of the derivatives with different hydrophobic properties have been shown.

[The influence of new hybrid antioxidants ichphans on the kinetics of ascorbate-induced reduction of spin probe radical centers in liposomes].

The influence of new hybrid antioxidants ichphans on the structure of liposome lipid bilayer was studied. The analysis of the kinetics of the ascorbate-induced reduction of spin probe radical centers incorporated in a membrane revealed that ichphans produce a modifying effect on the structure of liposome membrane. It was established that derivates with distinct hydrophobic properties transform different sites of the membrane that are the sites of localization of ichphans in the intramembraneous space.

[Interaction of nonelectrolytes, the derivatives of 5-hydroxybenzimidazole, with erythrocyte membrane].

The method of spin probe and scanning electron microscopy were used to study the effects of some new synthetic antioxidants and bioregulators, the derivatives of 5-hydroxybenzimidazole, on the membrane structure and morphology of erythrocytes. Analysis of EPR spectra and electron micrographs revealed that the derivatives with various side substituents affect the membrane structure and shape of erythrocytes in a concentration-dependent manner, the effect correlating with the hydrophobic properties of the side derivatives. It was shown that all the compounds in the concentration range 1.

[Influence of new hybrid antioxidants ichphans on erythrocyte morphology].

Scanning electron microscopy was used to study the effects of the new generation of compounds ICHFANs, which have a combined antioxidant and acetylcholine esterase inhibitory effect on the surface architectonics of erythrocytes. The incorporation of each of the studied compounds with the positively charged quaternary ammonium in the erythrocyte membrane and their distribution in the itramembraneous space were accompanied by the formation of echinocytes, stomatocytes, and compensative effects on erythrocyte shape. The time-dependent morphological transformation of erythrocytes apparently is determined by changes in the distribution of the compounds between the outer and inner monolayers of the erythrocyte membrane.