[Cytoplasmic enzyme release into the extracellular medium during the freezing and thawing of leukocytes].

The activity of cytoplasmic enzymes (hexokinase, phosphorylase, glucose-6-phosphate dehydrogenase, lactate dehydrogenase) in the extracellular medium following leucocyte freezing-thawing to -30, -70, -140 or -196 degrees C was studied. The appearance of these enzymes in the freezing medium has been found to be associated with the disruption of cellular elements. The source of lactate dehydrogenase activity in the extracellular medium was shown to be due not only to disrupted leucocytes, but also to cells remained after freezing-thawing.

[Effectiveness of cryogenic action in experimental liver resection].

The traditional ligature methods were added with freezing of the left hepatic lobe resection line in order to block up parenchymatous bleeding and cholerhagia in atypical resection of the liver. Alterations in the enzymatic activity (alaninic and asparaginic transaminases alkaline phosphotase, lactate dehydrogenase and ribonuclease) in the blood serum and liver of the rabbits allowed to judge about the character of inflammatory and destructive changes in the liver following the use of the abovementioned methods. The rise of the activity of the enzymes under study within the first days after operation and its normalization by the 7th to 14th days in all the studied variants of hemo- an cholestasis have been established.