A model of oxygen intake by aerobic bio-objects in liquid incubating media was applied to investigate the influence air-media interface area on accuracy of measuring the oxygen intake and error value. It was shown that intrusion of air oxygen increases the relative error to 24% in open polarographic cells and to 13% in cells with a reduced interface area. Results of modeling passive media oxygenation laid a basis for proposing a method to reduce relative error by 66% for open cells and by 15% for cells with a reduced interface area.
View Article and Find Full Text PDFThe content of polyamines in cadaveric bone marrow was measured before and after cryopreservation. The content of some polyamines was found to depend on the morphologic and functional preservation of bone marrow elements. It is concluded that the content of some polyamines may serve as criterion of cell destruction and proliferative potencies of bone marrow.
View Article and Find Full Text PDFContent of polyamines and some patterns of metabolism related to their synthesis and catabolism were studied in blood of patients with chronic myeloleukemia and chronic lympholeukemia. Free polyamines as well as activity of diamine oxidase were distinctly increased in blood, while concentration of total diamino acids was simultaneously decreased in blood serum of the patients as compared with that of healthy persons. Specific type of the quantitative alterations was responsible for efficiency of chemotherapy in these patients.
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