[The problems of a loss of activity of the sorbed ligands in heterogeneous immunoenzyme analysis].

Literature containing a number of data on the problem of the loss of activity of adsorbed ligands in the heterogeneous enzyme immunoassay has been analyzed. This analysis has demonstrated that this process is the superposition of the processes of both denaturation and oxidation of the adsorbed ligand, as well as its desorption from the surface of the plate. The proofs of both denaturation and desorption increments of the process of activity loss are presented.

[The use of modified polystyrene plates in solid-phase immunoenzyme analysis for the diagnosis of infections].

The practical aspects of the theory of the adsorption of bioligands on the plastic surface have been considered and discussed. The possibility of standardizing polystyrene plates, as well as obtaining plates with the predetermined level of adsorption activity with respect to given antigens (antibodies), has been shown with the use of the patented technology for the modification of polystyrene plates. The data on the adsorption of antigens used as an experimental model (human and rabbit immunoglobulin fractions) are presented, and an increase in adsorption activity has been shown with the use of modified plates.

[The use of the physicochemical laws governing the sorption properties of antigens (antibodies) in relation to the plastic solid phase for enhancing the sensitivity of immunoenzyme analysis test systems in the diagnosis of infections].

Some practical aspects of the theory of adsorption of bioligands on plastic surfaces have been considered and discussed. Modifications of experimental and actual antigens (antibodies) have been theoretically and practically realized on the concept of "solubility parameters" by means of pin-point hydrophobization. The use of this method has been shown to increase the sensitivity of individual ELISA systems based on rabbit and mouse immunoglobulins 1.

[The comparative characteristics of the peroxidase conjugates used in Soviet immunoenzyme test systems for detecting HIV antibodies].

Five lots of peroxidase conjugates were studied in enzyme immunoassay systems "Recombinant-HIV" and "Peptoscreen-2" for detection of antibody to HIV. The conjugates differed from each other by the source of generation and methods of preparation. The conjugates were studied in biochemical tests and ELISA.