[Effect of x-irradiation on the properties of cyclic AMP-dependent protein kinases from rat spleen lymphocytes].

It was shown, that in conditions of acute radiation affection in doze 0.5 and 1 Gy, there was a decrease of 32 and 55% in activity of cyclic AMP-dependent protein kinases, isolated from cytosol of lymphocytes. The analysis of cAMP-dependent protein kinases properties has shown, that disturbance in the interaction of the enzyme with protein substrate of phosphotransferase reaction and main modulater enzymes activity--cAMP proceeds of the ionising radiation effect.

[Activity and properties of cycl AMP-dependent protein kinases in the gray substance of the rat brain in acute radiation injury].

It was shown that in conditions of acute radiation affection there was a decrease of 37% and 32% in activity of cyclic AMP-dependent protein kinases, isolated from cytosol of gray substance of rat brain cells and from the synaptic membranes solubilized by triton X-100, respectively. The activity of enzymes from the control and irradiated animals was maximum with similar concentration of 3':5'-AMP (10(-6) M) and pH (7.4) in Tris-HCl and HEPES-buffers.