[Cloning of Shigella flexneri 2a genes coding for restriction- modification system Sfl2aI].

The Sfl2aI system of restriction-modification (RM) was revealed in the cells of Shigella flexneri encoded by pKMR114 plasmid belonging to the IncN incompatibility group. The genes for Sfl2aI RM system were cloned. The system was ascribed to the enzymes of the EcoRII specificity, as shown by in vivo and in vitro experiments.

[Restriction-modification systems of type II in Shigella strains].

Two restriction-modification systems specified by two plasmids are discovered in the clinical species of Shigella. The plasmids are designated pKMR114 and pKMR115. Both are of 60.