In vitro effects of folic acid on gamma-glutamyltransferase and glutathione reductase activities in malignant lung and thymus tumors.

In vitro effects of folic acid (10(-5), 10(-4), and 10(-3)M) on activities of gamma-glutamyltransferase and glutathione reductase, the enzymes involved in glutathione metabolism, were studied in tissue samples obtained after surgical treatment of the lungs and thymus. Folic acid did not change gamma-glutamyltransferase activity in lung cancer tissue, but in thymoma tissue this substance in a concentration of 10(-3)M inhibited it by 16%. Folic acid had no effects on glutathione reductase activity in benign tumors and normal lung and thymus tissues, but increased this activity in thymoma and lung cancer tissues.

[Suppurative-septic complications after surgical treatment of destructive pancreatitis].

Complex prophylactics of pyo-septic complications was used in 46 patients with acute destructive pancreatitis. It resulted in 11% less frequency of the complications, postoperative lethality became 28% lower. In 35 patients with the preserved spleen resection of the pancreas was followed by less lethality as compared with similar operations including splenectomy.

[Catabolism of native and modified high-density lipoproteins in rabbits].

Rates of fraction catabolism of rabbit and horse total 125I-high density lipoproteins (HDL) were shown to be similar in rabbits, after intravenous administration, while catabolism of human, dog and, especially, of rat lipoproteins occurred at the higher rate as compared with rabbit HDL. Rabbit HDL containing modified epsilon-aminogroups, or guanidine groups, or carboxyl groups and native HDL were quite similarly eliminated from circulation of healthy animals, whereas the lipoproteins with modified tyrosine residues and, especially, succinated HDL exhibited the highest rate of elimination. Radioactivity of spleen tissue and lymphatic glands was several-fold higher after intravenous administration of succinated homologous 125I-HDL into rabbits as compared with the effect of native homologous 125I-HDL; in kidney, adrenal glands, liver tissue and small intestine the level of radioactivity was analogous after treatment with both homologous native and succinated 125I-HDL.

[The rate of elimination of homologous and heterologous native and modified high density lipoproteins from rabbit plasma after their intravenous administration].

Rates of catabolism of total fraction of human native high density lipoproteins (HDL) and their subfractions as well as horse and rabbit native and modified HDL were studied after intravenous administration of the HDL into healthy rabbits and the animals with experimental hypercholesterolemia. The following procedures were carried out for production of HDL derivatives: methylation, succination, blocking of Arg residues and of COOH-groups. Rates of catabolism were similar for rabbit and horse total fractions of 125I-HDL, whereas human HDL were catabolized at a slightly higher rates as compared with rabbit lipoproteins.

[The rate of elimination of heterologous native and modified high-density lipoproteins from rabbit blood plasma after their intravenous administration].

Native and modified horse LPHD (methylated, succinylated, with blocked Arg-residues and COOH-groups) were administered intravenously (400 mg by protein) into healthy rabbits and into animals with hypercholesterolemia. Concentration of the modified LPHD decreased much more distinct in blood plasma of healthy rabbits as compared with native LPHD. The same results were obtained in rabbits with hypercholesterolemia after administration of methylated and succinylated LPHD.