[The isolation of the antigenic substances of Mycobacterium tuberculosis and the characteristics of their basic chemical components].

Different methods of isolation were used when obtaining the antigen determinants of the tuberculosis mycobacteria (tuberculins, polysaccharide, phosphatide). Main chemical components of the antigens were investigated. Most effective and economic methods of obtaining the M.

[The development of a latex antigenic diagnostic agent for the serological determination of tuberculous infection].

Using two tuberculoproteins, phosphatide and polysaccharide antigens obtained from culture filtrate of pathogenic and vaccinal strains of Mycobacterium of human and bull types and standard monodispersed particles of latex modified by different chemical groups, the technology of creation of latex antigenic preparations for serologic express diagnosis of tuberculosis was elaborated. This method is based on the reaction of latex agglutination. Latex diagnostic preparation possesses sufficiently high serologic activity and specificity.

[The determination of tetanus antitoxin by the latex agglutination reaction].

A new method to detect the protective value of antitetanic antibody in latex agglutination test (LAT) has been described. The new method is highly sensitive and fast, and requires no special equipment. The optimal conditions of sensibilization, latex types, test providing procedure have been selected.