Enhancing Sensitivity in Nucleic Acid Detection via Collaborative Multiple Catalytic Cores in DNAzyme Nanomachines.

We introduce a multicore DNA nanomachine (MDNM), utilizing four binary DNAzymes for nucleic acid detection without the need for a preamplification step. This innovation remarkably yields a reduction in limit of detection (LOD), over 5-fold, as compared to single-core systems. This reduces the required test time thus highlighting the potential of MDNM in advancing nucleic acid detection.

[Evaluation of the immunological activity and safety of group B meningococcal vaccine prepared from a natural complex of specific polysaccharide and outer membrane proteins].

Immunological activity and safety of group B meningococcal vaccine prepared from a natural complex of specific polysaccharide and outer membrane proteins were under study. The immunological safety of the vaccine was evaluated by the absence of antibodies to denaturated and native DNA (d-DNA and n-DNA). As shown with the use of the enzyme immunoassay (EIA), the administration of the vaccine did not induce antibody formation to d-DNA and n-DNA during the observation period.

[The isotypic characteristics of antibodies in persons inoculated with a serogroup-A meningococcal polysaccharide vaccine].

In this work the results of the study of specific antibodies (Ab), isotypes IgM, IgG, IgA, types kappa and lambda, in 235 serum samples from 27 adults immunized with group A meningococcal polysaccharide vaccine (AMPV) in a single injection of 50 microg and from 20 control subjects are presented. The study was made by the method of sandwich EIA. The study revealed that in a month after the injection of the vaccine the intensive synthesis of IgA, IgG and IgA Ab and their subsequent circulation for 2 years were observed; 3 years after immunization (the term of observation) the prevalence of IgG and IgA antibodies was registered.

[Immune bacteriolysis reaction in the assessment of immunological effectiveness of serogroup B meningococcal vaccine].

The immunological efficacy of serogroup B meningococcal protein-polysaccharide vaccine was studied in newly developed immune bacteriolysis test. Serogroup B meningococcus strains had different sensitivities to the bactericidal effects of immune sera. A single injection of the vaccine caused an induction of bactericidal antibodies to meningococcus vaccinal strain.

[Association of papillomavirus infection and latent hyperprolactinemia in patients with dysplasia and preinvasive cancer of the cervix uteri].

Gynecological screenings carried out in I, 247 females and supplemented by cytological examination of ectoendocervical mucosa smears established significantly higher rates of detection of cytological signs of papillomavirus infection (PVI), regardless of age, in cases of background pathologies of the uterine cervix (10.0%) than in females without detectable pathology of the ectocervix (3.6%).