Publications by authors named "L I Aref'eva"

Aim: Study sensitivity of nosocomial microbes--causative agents of post-operative infectious complications in surgical profile patients to bacteriophages.

Materials And Methods: Microbe isolates from biosubstrates of 223 surgical patients served as material. Microbes were isolated from blood, urine, wounds, intravascular catheters, abdominal cavity drainage, discharge of pharynx, trachea (343 strains).

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The morphology of female reproductive organs and anatomy, ultrastructure, and ultrasculpture of seed integuments were studied in more than 150 species of Podocarpales, Cephalotaxales, and Taxales. The main trends of morphological-evolutionary transformations of female fructifications were established in three studied groups of gymnosperms. The taxonomic weight of structural features of the female reproductive organs of Podocarpales, Cephalotaxales, and Taxales was determined.

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The patterns of protein biosynthesis in the embryo and endosperm during embryogenesis in the Scots pine were studied using electrophoresis and biochemistry methods. Proteins of the albumin-globulin fraction were visualized already at the early embryonic stages. The main polypeptide components (48-60, 37-39, and 20-22 kDa) were gradually accumulated in the course of maturation.

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The phylogenetic relationships of gymnospermous and angiospermous plants were studied. To this end, 13 antisera to seed proteins of plant taxa representing all the four classes of the gymnosperms were obtained. The antigens used in immunochemical reactions with these antisera included the proteins of 134 seed samples representing 91 families from all the 11 subclasses of dicotyledons and 64 seed samples representing 33 families from five out of six classes of monocotyledons (according to Takhtajan, 1996).

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Wheat prolamines were preincorporated into the endosperm of maize seed, and concentrations of free and bound amino acids were measured in the endosperm and seedlings during seed germination. Incorporation of foreign prolamines lowered the decrease rate of free proline concentration in both the endosperm (sprouting and shoots) and the embryo (sprouting). The seedlings at the stage of shoots were characterized by higher concentrations of aspartic acid, glutamic acid, and tyrosine.

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