Bioremediation of Oil-Contaminated Soil of the Republic of Kazakhstan Using a New Biopreparation.

A new biopreparation is developed to clean soils from oil pollution in the arid climate of the Republic of Kazakhstan. The biopreparation includes bacterial strains F2-1, F2-2, and BS3701. When using the biopreparation in a liquid mineral medium with 15% crude oil, laboratory studies have revealed degradation of 48% n-alkanes and 39% of PAHs after 50 days.

Use of natural sorbents for accelerated bioremediation of grey forest soil contaminated with crude oil.

Due to the extensive oil extraction and transportation that occurs in oil-producing countries, many lands remain contaminated because of accidental leakages. Despite its low cost and environmentally safe nature, bioremediation technology is not always successful, mainly because of the soil toxicity to the degrading microbial populations and plants. Here we report a three-year microfield experiment on the influence of natural sorbents of mineral (zeolite, kaolinite, vermiculite, diatomite), organic (peat), carbonaceous (biochar) origin, and a mixed sorbent ACD (composed of granular activated carbon and diatomite) on the bioremediation of grey forest soil contaminated with weathered crude oil (40.

Complete Genome Sequence of Pseudomonas putida BS3701, a Promising Polycyclic Aromatic Hydrocarbon-Degrading Strain for Bioremediation Technologies.

The strain BS3701 was isolated from soil contaminated with coke by-product waste (Moscow Region, Russian Federation). It is capable of degrading crude oil and polycyclic aromatic hydrocarbons (PAHs). The BS3701 genome consists of a 6,337,358-bp circular chromosome and two circular plasmids (pBS1141 with 107,388 bp and pBS1142 with 54,501 bp).

Microorganisms form exocellular structures, trophosomes, to facilitate biodegradation of oil in aqueous media.

Cytochemical staining and microscopy were used to study the trophic structures and cellular morphotypes that are produced during the colonization of oil-water interfaces by oil-degrading yeasts and bacteria. Among the microorganisms studied here, the yeasts (Schwanniomyces occidentalis, Torulopsis candida, Candida tropicalis, Candida lipolytica, Candida maltosa, Candida paralipolytica) and two representative bacteria (Rhodococcus sp. and Pseudomonas putida) produced exocellular structures composed of biopolymers during growth on petroleum hydrocarbons.

[Horizontal transfer of catabolic plasmids in the process of naphthalene biodegradation in model soil systems].

The process of naphthalene degradation by indigenous, introduced, and transconjugant strains was studied in laboratory soil microcosms. Conjugation transfer of catabolic plasmids was demonstrated in naphthalene-contaminated soil. Both indigenous microorganisms and an introduced laboratory strain BS394 (pNF142::TnMod-OTc) served as donors of these plasmids.