The H1 automated differential counter in determination of bone marrow remission in acute leukemia.

One hundred eighty-one bone marrow aspirates from 63 patients with acute leukemia were evaluated by the Technicon H1 automated differential counter and by microscopic examination for remission status after chemotherapy. Of the 163 marrows that were conclusive by microscopic examination, 130 were in remission (< 5% blast cells). This study focused on the following H1 alarms/flags to estimate their sensitivity for determination of remission: blast signal (BLSI), large unstained cells alarm (LUCA), baso blast alarm (BBLA), immature granulocytes (IGA) and atypia (ATYP).

Chronic B-cell leukemias: relation between morphological and immunological features.

To better understand the heterogeneity of chronic B-cell leukemias we correlated morphological and immunological features by studying the peripheral blood from 80 patients with a panel of anti-immunoglobulin and fourteen monoclonal antibodies, which hitherto were studied separately or with respect to one single morphological entity only. Of these the surface immunoglobulins (sIg) and monoclonal antibodies (McAb) BA-1, BA-2, FMC7, OKM1, and anti-T65 allowed a fair distinction between five cytological subtypes: chronic lymphocytic (CLL), "lymphoplasmacytoid" (LPL), centrocytic (CL), prolymphocytic (PLL), and hairy cell leukemia (HCL). In that order the sIg showed a decreasing number of cases of mu +/- delta class and an increase of alpha or gamma positivity.