Changes in the rates of lipogenesis and hepatic triglyceride secretion in mice bearing Ehrlich ascites carcinoma.

The growth of Ehrlich ascites tumors (EAT) in mice induces hypertriglyceridemia and depletion of lipid stores. H-Riop: Swiss mice in early and late stages of tumor growth were examined to investigate whether an increase in liver synthesis of fatty acids (FA) and/or an increase in the liver triglyceride (TG) secretion rates (TGSR) would contribute to endogenous cancer-induced hypertriglyceridemia. Using 3H2O as tracer, FA synthesis decreased in the liver of tumorous animals.

Thymidylate synthetase and thymidine kinase activities and methotrexate cytotoxicity during growth of L1210 and Ehrlich ascites tumor.

The specific activities of TS and TK determined in the cytosols of Ehrlich and L1210 ascites tumor cells changed significantly during their logarithmic growth. In both tumors, the highest activity of TK was in early log phase while that of TS occurred in late logarithmic growth. The activity curves of TS were practically the same and those of TK were similar in the two tumors; however, the absolute values of the latter were different.

Changes in the polyamine and nucleotide metabolism of P388 leukemia cells treated with DL-alpha-difluoromethylornithine in culture.

Influence of DL-alpha-difluoromethylornithine (DFMO) treatment on the growth kinetics, labelling index, extra- and intracellular polyamine and nucleotide concentrations was monitored in cultured P388 leukemia cells. A substantial decrease of cell proliferation was observed when the cells were continuously treated with 1-5 mM DFMO. Depletion of cellular polyamines, mostly of putrescine and spermidine, was seen with a concomitant but delayed increase of spermidine and spermine levels in the culture medium.

Permanent metachromatic effect of 1,9-dimethyl-methylene blue on acidic mucopolysaccharides of mastocytes.

The 1,9-dimethyl-methylene blue (SERVA) has a very strong metachromatic reaction with sulphated mucopolysaccharides of mastocytes at pH = 1 (Tris-HCl buffer). This metachromasia is retained also at increasing pH (2.5-5), and is not affected either by the quality or by the concentration of the ions present (1/240 M Tris-HCl, and 0.

Comparison of high-performance ion-exchange and ion-pair liquid chromatographic methods for the separation of tumour cell nucleotides.

Ion-exchange liquid chromatography (IELC) on a novel anion-exchanger, Polyanion SI HR 5/5, and the ion-pair technique (IPLC) using Hypersil ODS and/or MinoRPC reversed phases with tetrabutylammonium phosphate as pairing agent were compared for the separation of nucleotides. Modifications to the concentration gradient in IELC in the range 0.01-0.