Publications by authors named "L Hofman"
Objectives: After cleft lip and/or palate (CL/P) repair, children may develop velopharyngeal insufficiency (VPI) leading to speech imperfections, necessitating additional speech correcting surgery. This study examines the incidence of VPI and speech correcting surgery after Sommerlad's palatoplasty for CL/P, and its association with various clinical features.
Materials And Methods: A retrospective cohort study was performed in the Wilhelmina Children's Hospital in Utrecht and child records from 380 individuals with CL/P registered from 2008 to 2017 were retrospectively reviewed.
Objectives: In cleft palate patients, the soft palate is commonly closed using straight-line palatoplasty, Z-palatoplasty, or palatoplasty with buccal flaps. Currently, it is unknown which surgical technique is superior regarding speech outcomes. The aim of this review is to study the incidence of speech correcting surgery (SCS) per soft palatoplasty technique and to identify variables which are associated with this outcome.
View Article and Find Full Text PDFCRISPR-Cas enzymes enable RNA-guided bacterial immunity and are widely used for biotechnological applications including genome editing. In particular, the Class 2 CRISPR-associated enzymes (Cas9, Cas12 and Cas13 families), have been deployed for numerous research, clinical and agricultural applications. However, the immense genetic and biochemical diversity of these proteins in the public domain poses a barrier for researchers seeking to leverage their activities.
View Article and Find Full Text PDFArticle Synopsis
- CRISPR-Cas9 genome editing is advancing T cell therapies, but there's a concern about the loss of targeted chromosomes, which could impact safety.
- A study showed that chromosome loss is widespread in primary human T cells and can occur with both partial and complete chromosome loss, even in preclinical therapies.
- The researchers developed a modified manufacturing process that reduces chromosome loss while maintaining the effectiveness of genome editing, finding that p53 expression might help protect against this issue in clinical applications.