Fast and sensitive HPLC method for the determination of neopterin, kynurenine and tryptophan in amniotic fluid, malignant effusions and wound exudates.

Aim: A new HPLC method for the determination of neopterin, kynurenine and tryptophan using a second-generation monolith stationary phase and high-throughput sample preparation procedure based on microplates was developed and fully validated.

Materials & Methods: As the stationary phase a monolithic C18 Chromolith high-resolution column with dimensions of 4.6 × 100 mm connected to a monolithic 4.

[Psychosomatic aspects and antipsychotics medication in ethiopathogenesis of endometrial carcinoma].

Objective: To assess possible role of psychosomatic aspects and antipsychotic medical treatment in ethio-pathogenesis of endometrial cancer.

Design: Prospective study and revue of literature.

Setting: Department of Obstetrics and Gynecology, Faculty of Medicine and Dentistry, University Hospital, Olomouc; Department of Health Care Science, Faculty of Humanities, Tomas Bata University in Zlín.

[Gynaecological surgery of Jehovah´s Witnesses].

Objective: To present experience with surgical treatment of various gynaecological diseases in patients belonging to the Church of Jehova´s Witness. DESIGNE: Retrospective study.

Setting: Department of Obstetrics and Gynaecology, Palacky University Olomouc, Institute of Health Care Studies, Faculty of Humanity Studies, Thomas Bata University, Zlín.

[Psychosomatic aspects of malignant tumours of uterine corpus].

Objective: Evaluation of selected scales of the Minnesota Multiphasis Personality Inventory-2 (MMPI-2) within the frame of psychosomatic aspects of gynecological oncology.

Design: Assessment of actual emotional state and its comparison with the general personality traits of patients before a surgery for gynecologic malignancy.

Setting: Department of Obstetrics and Gynecology, University Hospital Medical Faculty PU, Olomouc.

[Determination of beta 2 microglobulin in malignant tumors of the ovaries].

Beta 2-microglobulin (beta 2-MG) is used as a tumour marker in some malignant diseases. The authors assessed the concentration of beta 2-MG in serum of 30 patients with malignant ovarian tumours and in 29 patients with other benign gynaecological diseases, in particular benign ovarian tumours. Although raised values of beta 2-MG were found in ovarian carcinomas, as compared with the control group of non-malignant gynaecological diseases, a statistically significant increase was recorded only in carcinomas grade III and IV.