Introduction: The gene cluster, encoding the sole iron-sulfur (Fe-S) cluster assembly system in , was recently shown to be up-regulated in response to oxidative stressors and Fe limitation.
Methods: In this study, luciferase reporter fusion assays, electrophoretic gel mobility shift assays (EMSA) and transcription assays (IVT) were used to dissect the and acting factors that regulate the expression of .
Results And Discussion: Results showed deletion of , for the only Fur-family transcriptional regulator in , resulted in >5-fold increases in luciferase activity under the control of the promoter (P<0.
Estrogen receptors (ER) are widely expressed in the brain of many species and experimental results highlighted the role of estradiol in neuronal plasticity and behavior. Consequently, the brain is therefore a prime target for endocrine disrupting chemicals (EDCs) interacting with estrogen signaling. Very little is known about the late effects of early disruption of estrogen signaling by EDCs.
View Article and Find Full Text PDFPseudomonas aeruginosa (Pa) and Methicillin susceptible Staphylococcus aureus (MSSA) are the predominant bacteria found in the airways of people with cystic fibrosis (pwCF), significantly contributing to lung disease progression. While various factors influencing the initial acquisition (IA) of these pathogens are known, the impact of environmental conditions remains understudied. This epidemiological study assessed the risk of MSSA and Pa initial acquisitions in relation to seasonality and climatic zones among 1,184 French pwCF under 18 years old.
View Article and Find Full Text PDFBackground: is a common pathogen that contributes to progressive lung disease in cystic fibrosis (CF). Genetic factors other than CF-causing (CF transmembrane conductance regulator) variations contribute ∼85% of the variation in chronic infection age in CF according to twin studies, but the susceptibility loci remain unknown. Our objective is to advance understanding of the genetic basis of host susceptibility to infection.
View Article and Find Full Text PDFAirway epithelial cells form a physical barrier against inhaled pathogens and coordinate innate immune responses in the lungs. Bronchial cells in people with cystic fibrosis (pwCF) are colonized by Pseudomonas aeruginosa because of the accumulation of mucus in the lower airways and an altered immune response. This leads to chronic inflammation, lung tissue damage, and accelerated decline in lung function.
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