A high throughput array microhabitat platform reveals how light and nitrogen colimit the growth of algal cells.

A mechanistic understanding of algal growth is essential for maintaining a sustainable environment in an era of climate change and population expansion. It is known that algal growth is tightly controlled by complex interactive physical and chemical conditions. Many mathematical models have been proposed to describe the relation of algal growth and environmental parameters, but experimental verification has been difficult due to the lack of tools to measure cell growth under precise physical and chemical conditions.

Colimitation of light and nitrogen on algal growth revealed by an array microhabitat platform.

Microalgae are key players in the global carbon cycle and emerging producers of biofuels. Algal growth is critically regulated by its complex microenvironment, including nitrogen and phosphorous levels, light intensity, and temperature. Mechanistic understanding of algal growth is important for maintaining a balanced ecosystem at a time of climate change and population expansion, as well as providing essential formulations for optimizing biofuel production.

The tumor stroma influences immune cell distribution and recruitment in a PDAC-on-a-chip model.

The dense tumor stroma of pancreatic ductal adenocarcinoma (PDAC) and its secreted immune active molecules provide a barrier for chemotherapy treatment as well as for immune cell infiltration to the tumor core, providing a challenge for immunotherapeutic strategies. Consequently, the investigation of processes underlying the interaction between the tumor stroma, particularly activated pancreatic stellate cells (PSCs), and immune cells may offer new therapeutic approaches for PDAC treatment. In this study, we established a 3D PDAC model cultured under flow, consisting of an endothelial tube, PSCs and PDAC organoids.

A microfluidic-based PDAC organoid system reveals the impact of hypoxia in response to treatment.

Pancreatic Ductal Adenocarcinoma (PDAC) is estimated to become the second leading cause of cancer-related deaths by 2030 with mortality rates of up to 93%. Standard of care chemotherapeutic treatment only prolongs the survival of patients for a short timeframe. Therefore, it is important to understand events driving treatment failure in PDAC as well as identify potential more effective treatment opportunities.

Practical and thermodynamic constraints on electromicrobially accelerated CO mineralization.

By the end of the century, tens of gigatonnes of CO will need to be removed from the atmosphere every year to maintain global temperatures. Natural weathering of ultramafic rocks and subsequent mineralization reactions can convert CO into ultra-stable carbonates. Although this will draw down all excess CO, it will take thousands of years.