The secreted tyrosine phosphatase PtpA promotes survival in RAW 264.7 macrophages through decrease of the SUMOylation host response.

uses numerous strategies to survive and persist in the intracellular environment of professional phagocytes, including modulation of the SUMOylation process. This study aims to understand how alters host SUMOylation to enhance its intracellular survival in professional phagocytes. Our results indicate that strain Newman utilizes PtpA-driven phosphorylation to decrease the amount of SUMOylated proteins in murine macrophages to facilitate its survival in this immune cell type.

Characterization of the Secreted Acid Phosphatase SapS Reveals a Novel Virulence Factor of That Contributes to Survival and Virulence in Mice.

possesses a large arsenal of immune-modulating factors, enabling it to bypass the immune system's response. Here, we demonstrate that the acid phosphatase SapS is secreted during macrophage infection and promotes its intracellular survival in this type of immune cell. In animal models, the SA564 mutant demonstrated a significantly lower bacterial burden in liver and renal tissues of mice at four days post infection in comparison to the wild type, along with lower pathogenicity in a zebrafish infection model.

Decreases SUMOylation Host Response to Promote Intramacrophage Survival.

is a commensal bacterium that causes severe infections in soft tissue and the bloodstream. During infection, manipulates host cell response to facilitate its own replication and dissemination. Here, we show that significantly decreases the level of SUMOylation, an essential post-translational modification, in infected macrophages 24 h post-phagocytosis.

The secreted protein kinase CstK from influences vacuole development and interacts with the GTPase-activating host protein TBC1D5.

The intracellular bacterial pathogen is the etiological agent of the emerging zoonosis Q fever. Crucial to its pathogenesis is type 4b secretion system-mediated secretion of bacterial effectors into host cells that subvert host cell membrane trafficking, leading to the biogenesis of a parasitophorous vacuole for intracellular replication. The characterization of prokaryotic serine/threonine protein kinases in bacterial pathogens is emerging as an important strategy to better understand host-pathogen interactions.