One-Step Purification of Microbially Produced Hydrophobic Terpenes via Process Chromatography.

Novel and existing terpenes are already being produced by genetically modified microorganisms, leading to new process challenges for the isolation and purification of these terpenes. Here, eight different chromatographic resins were characterized for the packed bed adsorption of the model terpene β-caryophyllene, showing their applicability on an fermentation mixture. The polystyrenic Rensa RP (Ø 50 μm) shows the highest affinity, with a maximum capacity of >100 g L and the best efficiency, with a height equivalent of a theoretical plate (HETP) of 0.

Membrane-assisted extraction of monoterpenes: from solvent screening towards biotechnological process application.

This work focuses on the process development of membrane-assisted solvent extraction of hydrophobic compounds such as monoterpenes. Beginning with the choice of suitable solvents, quantum chemical calculations with the simulation tool COSMO-RS were carried out to predict the partition coefficient (log) of (S)-(+)-carvone and terpinen-4-ol in various solvent-water systems and validated afterwards with experimental data. COSMO-RS results show good prediction accuracy for non-polar solvents such as n-hexane, ethyl acetate and n-heptane even in the presence of salts and glycerol in an aqueous medium.

[Pathomorphologic and ultrastructural characteristics of endocardial Cysticercus cellulosae].

A case is presented of a solitary endocardial cysticercus of cellulose discovered at a biopsy in the left auricula of the heart, got by a 36-year old patient at the operation who had a mitral and aortal defect caused by a reumatic endocarditis. The cisticercus is implanted on the surface of an intact endothelium and the fibroelastic structure of the superficial part of the endocardium is preserved. Inflammation of the endocardium and the myocardium is presented by a perivascular infiltrates eosinophile granulocytes, plasms cells and lymphocytes, suggesting an allergic nature of inflammation.