[Immunoenzyme analysis of post-vaccination secretory immunity to influenza A and B viruses using a manufactured monoclonal immunoenzyme test system].

The formation of postvaccinal secretory immunity to influenza A and B viruses was studied by a new monoclonal enzyme immunoassay test system for measuring specific secretory IgA in young people vaccinated with live cold-adapted vaccines (LCAV) intranasally and with inactivated commercial centrifuged influenza vaccine (IIV) parenterally, intranasally, and orally. Secretory IgA most intensively accumulated in subjects intranasally vaccinated with LCAV, less so in subjects vaccinated with IIV intranasally and orally, and just negligibly in those vaccinated with IIV parenterally. In vaccinees immunized with LCAV intranasally the intensity of immune secretory response depended on the initial concentrations of specific IgA before vaccination.

[Monoclonal immunoenzyme test-system for evaluating secretory immunity to influenza A and B viruses].

Enzyme immunoassay system has been developed for measuring the titers of secretory IgA in secretion from the upper respiratory airways (SURA). Russian ingredients are used. The conjugate includes monoclonal antibodies to heavy chains of human secretory IgA.

[The frequency of antibodies against delta virus in patients with HBs positive hepatitis].

Patients with HBsAg-positive hepatitis were screened for antibodies to HDV and HCV. The incidence of anti-HDV was 12.5% in a sample of 265 patients.

[Significance of the functional activity of antibodies in influenza immunity].

A simple and inexpensive test for mass examination of the functional activity of serum antibodies was developed. The test is based on a kinetic serologic reaction that reflects the time course of changes in antibody titers depending on the time of contact of the tested material with antigen. The curves of serum kinetic titration were processed on a computer by the special programme.

[Antibody functional activity in immunization with influenza vaccines].

The functional activity of serum antibody had been shown previously to reflect the state of resistance to influenza more accurately than antibody concentration. The functional activity of antibodies in the vaccination process was studied. The immune response to influenza antigens was found to be accompanied by an increase in the functional activity of antibodies, the intensity of this increase was the highest in natural influenza infection, moderate after administration of live vaccines and the least after immunization with inactivated preparations.