[Ultrastructure of dendritic spines in the somatosensory cortex of rat's brain following 5- and 33-day hypergravity].

Electron microscopy examination of layers III-IV in the somatosensory cortex of rat's brain following 5- and 33-day and repeated 5-day exposure to 2 G revealed ultrastructural changes in dendritic spines, synapses, axon terminals and nervous cell body suggesting their functional activation and increase of the afferent input from hindlimb mechanoreceptors Hypertrophy of the acicular apparatus (AA) due to hypergravity, growth of AA destruction as a result of 33-day exposure and its ample evidence after repeated 5-day exposure lead to the conclusion that AA ultrastructure can be used for assessing afferent input into the brain cortex of animals exposed in a changed field of gravity.

[Change in formation of the somatosensory cortex ultrastructure in rats due to hypergravity in pre- and postnatal ontogenesis].

Chronic action of gravity force of 2 G on pre- and postnatal rats up to day 75 of postnatal development gave rise to ultrastructural deviations in the somatosensory cortex (SSC) suggesting higher afferentation to SSC as compared with their counterparts at 1 g, more intensive synaptic transmission, afferent input by dendrites and neurons functioning with more frequent interneuronic contacts, glia activation and increased blood supply that altogether pointed to a permanent impact of elevated extero- and proprioceptive input throughout the time of SSC formation.

[Autocrine factor participation in defence of cytotoxic CTLL-2 cells from oxidative stress].

The role of autocrine factors (AF) secreted by cytotoxic IL-2-dependent CTLL-2 cells along with pyruvate in cell defense from oxidative stress was investigated. The addition of conditioned medium (CM) containing pyruvate and AF into CTLL-2 cell cultures increased significantly cell survival under oxidative stress condition. The kinetics of hydrogen peroxide removal from cell cultures under oxidative stress in the case of CM addition has been obtained.

[Identification and expression of haponin, a new protein from HL-60 cells].

We found a new protein haponin (an HLDF-alike protein) in promyelocyte HL-60 cells that is immunoreactive to polyclonal antibodies against HLDFbeta. Determination of the partial primary structure of the protein allowed us to reveal an immunogenic peptide of haponin and, on the basis of the amino acid sequence of this peptide, the degenerate primers were synthesized, which enabled us to clone the full-size cDNA of haponin. The stable heterologous expression of this cDNA in E.

[Primary and repeated exposure to hypergravity: ultrastructure of the somatosensory cortex in rats].

Nineteen-day centrifugation of rats at 2 G and ensuing 30-d readaptation to 1 g caused changes in the somatosensory cortex (SSC) ultrastructure pointing to intensification of the afferent input and functional activity of SSC neurons during hypergravity, and recovery of these processes in the course of readaptation. Repeated 5-d centrifugation of the rats at 2 G produced changes in ultrastructure of SSC layers 3 and 4 evidencing a more effective cortex functioning aimed at facilitation of adaptation to hypergravity repeated after the lengthy delay.