[Effectiveness of the oral administration of tomicide in experimental infection].

Experimental data on the oral administration of Tomicid using different schemes for the protection of white mice from staphylococcal infection are presented. The use of Tomicid, administered in the maximum dose admissible for mice, ensured the protection of up to 2/3 of the total number of mice. A single oral administration of the preparation immediately after infection protected 1/3 of the survived mice from local staphylococcal infection.

[Cultivation of Pseudomonas aeruginosa in media with chlorella fermentative hydrolysate as nutrient base].

The specific features of the growth of P. aeruginosa in media prepared with the use of Chlorella fermentative hydrolysate as nutrient basis were studied. The accumulation of microbial biomass was shown to depend on increased content of amino nitrogen in the media, while the effectiveness of the pyocyanin formation was linked with decreased content of amino nitrogen and a rise in the amount of sodium chloride.

[The action of tomicide on macromolecular synthesis in bacterial cells].

The study of the rate of incorporation of labeled precursors for nucleic acids and protein into Staphylococcus aureus 209 P cell fraction, insoluble in trichloroacetic acid, has revealed that in the presence of tomicide in the medium in a dose of 1 MCI (600 micrograms/ml) the synthesis of DNA in inhibited rapidly and almost completely (by 90%). The inhibition of the rate of incorporation of 3H-thymidine into the cells of staphylococcal culture by tomicide directly correlates with the concentration of the preparation within the range 100-600 micrograms/ml, the inhibition of the synthesis of RNA and protein being less pronounced than the inhibition of the synthesis of DNA.

[Tomicide adsorption on bacterial cells].

The capacity of the bacteriocin-like factor of tomicid being adsorbed on microbial cells, depending on the sensitivity of test microorganisms to the preparation, its form and concentration, the duration of the contact of microbial cells with tomicid, the temperature and pH of the incubation medium, was studied. The bacteriocin-like factor of tomicid was found to be capable of nonspecific sorption on microbial cells (in an amount of 64-128 units per mg of dried cell mass). The decrease of the temperature of the incubation medium to 0 degrees C and its pH to 4.

[Experimental study of the immunostimulating properties of tomitsid].

Tomicide, a preparation with antibacterial properties, accumulates in the culture medium during the growth of one of streptococcal strains. The injection of the preparation into mice simultaneously with the antigen (sheep red blood cells) produces an adjuvant effect. Tomicide has been found capable of inducing the production of interferon detected in the serum of the animals.