Non-invasive real-time investigation of colorectal cells tight junctions by Raman microspectroscopy analysis combined with machine learning algorithms for organ-on-chip applications.

Introduction: Colorectal cancer is the third most common malignancy in developed countries. Diagnosis strongly depends on the pathologist's expertise and laboratory equipment, and patient survival is influenced by the cancer's stage at detection. Non-invasive spectroscopic techniques can aid early diagnosis, monitor disease progression, and assess changes in physiological parameters in both heterogeneous samples and advanced platforms like Organ-on-Chip (OoC).

Aluminum Nitride Thin Film Piezoelectric Pressure Sensor for Respiratory Rate Detection.

In this study, we propose a low-cost piezoelectric flexible pressure sensor fabricated on Kapton (™ ) substrate by using aluminum nitride (AlN) thin film, designed for the monitoring of the respiration rate for a fast detection of respiratory anomalies. The device was characterized in the range of 15-30 breaths per minute (bpm), to simulate moderate difficult breathing, borderline normal breathing, and normal spontaneous breathing. These three breathing typologies were artificially reproduced by setting the expiratory to inspiratory ratios (E:I) at 1:1, 2:1, 3:1.

Label-free electrochemical biosensor for direct detection of Oncostatin M (OSM) inflammatory bowel diseases (IBD) biomarker in human serum.

Oncostatin M (OSM) is an interleukin-6 (IL-6) member family cytokine implicated in the pathogenesis of chronic diseases including inflammatory bowel disease (IBD). OSM is a novel diagnostic biomarker over-expressed in the serum of IBD patients. This paper reports on the first electrochemical OSM immunosensor, developed using a multistep fabrication process aimed at covalently immobilizing OSM antibodies on a mixed self-assembled monolayer coated gold working electrode.

Acoustic Waves in Piezoelectric Layered Structure for Selective Detection of Liquid Viscosity.

The acoustic waves of higher orders propagating in a layered structure consisting of a silicon plate coated with piezoelectric and/or films were used for the development of a sensor with selective sensitivity to liquid viscosity in the range of 1-1500 cP. In that range, this sensor possessed low sensitivity to liquid conductivity and temperature T in the ranges of 0-2 S/m and 0-55 °C, respectively. The amplitude responses insensitive to the temperature instead of the phase were used to provide the necessary selectivity.

TEER and Ion Selective Transwell-Integrated Sensors System for Caco-2 Cell Model.

Monitoring of ions in real-time directly in cell culture systems and in organ-on-a-chip platforms represents a significant investigation tool to understand ion regulation and distribution in the body and ions' involvement in biological mechanisms and specific pathologies. Innovative flexible sensors coupling electrochemical stripping analysis (square wave anodic stripping voltammetry, SWASV) with an ion selective membrane (ISM) were developed and integrated in Transwell™ cell culture systems to investigate the transport of zinc and copper ions across a human intestinal Caco-2 cell monolayer. The fabricated ion-selective sensors demonstrated good sensitivity (1 × 10 M ion concentration) and low detection limits, consistent with pathophysiological cellular concentration ranges.